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antibody against human ago2  (Millipore)


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    Structured Review

    Millipore antibody against human ago2
    Antibody Against Human Ago2, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/antibody+against+human+ago2/anti+ago2+antibody/pm37568709-94-17-21
    Average 90 stars, based on 1 article reviews
    antibody against human ago2 - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Lysis:

    Article Title: Silencing of circTASP1 inhibits proliferation and induces apoptosis of acute myeloid leukaemia cells through modulating miR‐515‐5p/HMGA2 axis
    Article Snippet: EZ‐magna RIP RNA‐binding protein immunoprecipitation kit (Millipore) was used for RIP detection to investigate the interaction between circTASP1 and mir‐515‐5p. .. After lysis of cells, the cells were incubated with Millipore antibody against human Ago2 coated on magnetic beads in RIP buffer solution. ..

    Article Title: Long Intergenic Nonprotein Coding RNA 00174 Aggravates Lung Squamous Cell Carcinoma Progression via MicroRNA-185-5p/Nuclear Factor IX axis
    Article Snippet: Recombinant luciferase reporter vectors LINC00174 WT/MUT and AZIN1 3′UTR WT/MUT were cotransfected with miR-185-5p mimics or NC mimics into SK-MES-1 or NCI-H520 cells and then analyzed after 48 h by Dual-Luciferase Reporter Assay System (Promega). .. The lysates of 1 × 10 7 LUSC cells were acquired from RIP lysis buffer for immunoprecipitation with the antibody against human Ago2 or normal control IgG (Millipore, Bedford, MA) for 1 h. After incubation with beads, the purified RNAs were subjected to RT-qPCR analysis. .. The lysates of 1 × 10 7 LUSC cells were acquired from RIP lysis buffer for immunoprecipitation with the antibody against human Ago2 or normal control IgG (Millipore, Bedford, MA) for 1 h. After incubation with beads, the purified RNAs were subjected to RT-qPCR analysis.

    Incubation:

    Article Title: Silencing of circTASP1 inhibits proliferation and induces apoptosis of acute myeloid leukaemia cells through modulating miR‐515‐5p/HMGA2 axis
    Article Snippet: EZ‐magna RIP RNA‐binding protein immunoprecipitation kit (Millipore) was used for RIP detection to investigate the interaction between circTASP1 and mir‐515‐5p. .. After lysis of cells, the cells were incubated with Millipore antibody against human Ago2 coated on magnetic beads in RIP buffer solution. ..

    Article Title: Long Intergenic Nonprotein Coding RNA 00174 Aggravates Lung Squamous Cell Carcinoma Progression via MicroRNA-185-5p/Nuclear Factor IX axis
    Article Snippet: Recombinant luciferase reporter vectors LINC00174 WT/MUT and AZIN1 3′UTR WT/MUT were cotransfected with miR-185-5p mimics or NC mimics into SK-MES-1 or NCI-H520 cells and then analyzed after 48 h by Dual-Luciferase Reporter Assay System (Promega). .. The lysates of 1 × 10 7 LUSC cells were acquired from RIP lysis buffer for immunoprecipitation with the antibody against human Ago2 or normal control IgG (Millipore, Bedford, MA) for 1 h. After incubation with beads, the purified RNAs were subjected to RT-qPCR analysis. .. The lysates of 1 × 10 7 LUSC cells were acquired from RIP lysis buffer for immunoprecipitation with the antibody against human Ago2 or normal control IgG (Millipore, Bedford, MA) for 1 h. After incubation with beads, the purified RNAs were subjected to RT-qPCR analysis.

    Magnetic Beads:

    Article Title: Silencing of circTASP1 inhibits proliferation and induces apoptosis of acute myeloid leukaemia cells through modulating miR‐515‐5p/HMGA2 axis
    Article Snippet: EZ‐magna RIP RNA‐binding protein immunoprecipitation kit (Millipore) was used for RIP detection to investigate the interaction between circTASP1 and mir‐515‐5p. .. After lysis of cells, the cells were incubated with Millipore antibody against human Ago2 coated on magnetic beads in RIP buffer solution. ..

    Article Title: Hsa_circ_0004370 promotes esophageal cancer progression through miR-1294/LASP1 pathway
    Article Snippet: Subsequently, cell lysis and RIP assay were performed by Magna RIP 134 RNA-Binding Protein Immunoprecipitation Kit (Merk Millipore, Darmstadt, Germany) as manual 135 described. .. The magnetic beads were precoated with antibody against human Ago2 (Millipore) and 136 antibody against IgG (Millipore) was set as an input control. ..

    Control:

    Article Title: Hsa_circ_0004370 promotes esophageal cancer progression through miR-1294/LASP1 pathway
    Article Snippet: Subsequently, cell lysis and RIP assay were performed by Magna RIP 134 RNA-Binding Protein Immunoprecipitation Kit (Merk Millipore, Darmstadt, Germany) as manual 135 described. .. The magnetic beads were precoated with antibody against human Ago2 (Millipore) and 136 antibody against IgG (Millipore) was set as an input control. ..

    Article Title: Long Intergenic Nonprotein Coding RNA 00174 Aggravates Lung Squamous Cell Carcinoma Progression via MicroRNA-185-5p/Nuclear Factor IX axis
    Article Snippet: Recombinant luciferase reporter vectors LINC00174 WT/MUT and AZIN1 3′UTR WT/MUT were cotransfected with miR-185-5p mimics or NC mimics into SK-MES-1 or NCI-H520 cells and then analyzed after 48 h by Dual-Luciferase Reporter Assay System (Promega). .. The lysates of 1 × 10 7 LUSC cells were acquired from RIP lysis buffer for immunoprecipitation with the antibody against human Ago2 or normal control IgG (Millipore, Bedford, MA) for 1 h. After incubation with beads, the purified RNAs were subjected to RT-qPCR analysis. .. The lysates of 1 × 10 7 LUSC cells were acquired from RIP lysis buffer for immunoprecipitation with the antibody against human Ago2 or normal control IgG (Millipore, Bedford, MA) for 1 h. After incubation with beads, the purified RNAs were subjected to RT-qPCR analysis.

    other:

    Article Title: Hsa_circ_0004370 promotes esophageal cancer progression through miR-1294/LASP1 pathway
    Article Snippet: Subsequently, cell lysis and RNA immunoprecipitation (RIP) assay were performed by Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Merk Millipore, Darmstadt, Germany) as described in manual.

    Transfection:

    Article Title: Functional In Vivo Screening Identifies microRNAs Regulating Metastatic Dissemination of Prostate Cancer Cells to Bone Marrow.
    Article Snippet: .. In brief, cells were harvested 48 h after transfection with miR-379-5p mimics or negative control, and the antibody against human AGO2 (Sigma Aldrich, Saint Louise, MO, USA) was used. ..

    Article Title: Functional In Vivo Screening Identifies microRNAs Regulating Metastatic Dissemination of Prostate Cancer Cells to Bone Marrow
    Article Snippet: .. In brief, cells were harvested 48 h after transfection with miR-379-5p mimics or negative control, and the antibody against human AGO2 (Sigma Aldrich, Saint Louise, MO, USA) was used. ..

    Negative Control:

    Article Title: Functional In Vivo Screening Identifies microRNAs Regulating Metastatic Dissemination of Prostate Cancer Cells to Bone Marrow.
    Article Snippet: .. In brief, cells were harvested 48 h after transfection with miR-379-5p mimics or negative control, and the antibody against human AGO2 (Sigma Aldrich, Saint Louise, MO, USA) was used. ..

    Article Title: Functional In Vivo Screening Identifies microRNAs Regulating Metastatic Dissemination of Prostate Cancer Cells to Bone Marrow
    Article Snippet: .. In brief, cells were harvested 48 h after transfection with miR-379-5p mimics or negative control, and the antibody against human AGO2 (Sigma Aldrich, Saint Louise, MO, USA) was used. ..

    Immunoprecipitation:

    Article Title: Long Intergenic Nonprotein Coding RNA 00174 Aggravates Lung Squamous Cell Carcinoma Progression via MicroRNA-185-5p/Nuclear Factor IX axis
    Article Snippet: Recombinant luciferase reporter vectors LINC00174 WT/MUT and AZIN1 3′UTR WT/MUT were cotransfected with miR-185-5p mimics or NC mimics into SK-MES-1 or NCI-H520 cells and then analyzed after 48 h by Dual-Luciferase Reporter Assay System (Promega). .. The lysates of 1 × 10 7 LUSC cells were acquired from RIP lysis buffer for immunoprecipitation with the antibody against human Ago2 or normal control IgG (Millipore, Bedford, MA) for 1 h. After incubation with beads, the purified RNAs were subjected to RT-qPCR analysis. .. The lysates of 1 × 10 7 LUSC cells were acquired from RIP lysis buffer for immunoprecipitation with the antibody against human Ago2 or normal control IgG (Millipore, Bedford, MA) for 1 h. After incubation with beads, the purified RNAs were subjected to RT-qPCR analysis.

    Purification:

    Article Title: Long Intergenic Nonprotein Coding RNA 00174 Aggravates Lung Squamous Cell Carcinoma Progression via MicroRNA-185-5p/Nuclear Factor IX axis
    Article Snippet: Recombinant luciferase reporter vectors LINC00174 WT/MUT and AZIN1 3′UTR WT/MUT were cotransfected with miR-185-5p mimics or NC mimics into SK-MES-1 or NCI-H520 cells and then analyzed after 48 h by Dual-Luciferase Reporter Assay System (Promega). .. The lysates of 1 × 10 7 LUSC cells were acquired from RIP lysis buffer for immunoprecipitation with the antibody against human Ago2 or normal control IgG (Millipore, Bedford, MA) for 1 h. After incubation with beads, the purified RNAs were subjected to RT-qPCR analysis. .. The lysates of 1 × 10 7 LUSC cells were acquired from RIP lysis buffer for immunoprecipitation with the antibody against human Ago2 or normal control IgG (Millipore, Bedford, MA) for 1 h. After incubation with beads, the purified RNAs were subjected to RT-qPCR analysis.

    Quantitative RT-PCR:

    Article Title: Long Intergenic Nonprotein Coding RNA 00174 Aggravates Lung Squamous Cell Carcinoma Progression via MicroRNA-185-5p/Nuclear Factor IX axis
    Article Snippet: Recombinant luciferase reporter vectors LINC00174 WT/MUT and AZIN1 3′UTR WT/MUT were cotransfected with miR-185-5p mimics or NC mimics into SK-MES-1 or NCI-H520 cells and then analyzed after 48 h by Dual-Luciferase Reporter Assay System (Promega). .. The lysates of 1 × 10 7 LUSC cells were acquired from RIP lysis buffer for immunoprecipitation with the antibody against human Ago2 or normal control IgG (Millipore, Bedford, MA) for 1 h. After incubation with beads, the purified RNAs were subjected to RT-qPCR analysis. .. The lysates of 1 × 10 7 LUSC cells were acquired from RIP lysis buffer for immunoprecipitation with the antibody against human Ago2 or normal control IgG (Millipore, Bedford, MA) for 1 h. After incubation with beads, the purified RNAs were subjected to RT-qPCR analysis.



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    Danaher Inc antibody against human ago2
    miR-640 was downregulated in RCC and correlated with circ_0054537 via targeting. (a, b) GSE61741 compared miRNAs levels including miR-640 in peripheral bloods between renal cancer patients and normal controls. (c, e) RT-qPCR detected miR-640 level in 39 RCC patients’ tissues and cells (HK-2, 786-O and A498). (d) Pearson’s correlation analysis analyzed the correlation between circ_0054537 and miR-640 levels in RCC tissues. (f) The predicted miR-640-binding sites in circ_0054537-WT were mutated. (g, h) Dual-luciferase reporter assay system compared luciferase activity of circ_0054537-WT/MUT vectors between miR-con mimic (miR-con) transfection and miR-640 mimic (miR-640) transfection in 786-O and A498 cells. (i, j) RIP assay detected RNA enrichment of circ_0054537 and miR-640 between <t>anti-Ago2-mediated</t> complex and anti-IgG-mediated complex. (k) RT-qPCR compared miR-640 level between si-con-transfected 786-O and A498 cells and si-circ_0054537 #1 -transfected above cells. *** P < 0.001
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    Image Search Results


    miR-640 was downregulated in RCC and correlated with circ_0054537 via targeting. (a, b) GSE61741 compared miRNAs levels including miR-640 in peripheral bloods between renal cancer patients and normal controls. (c, e) RT-qPCR detected miR-640 level in 39 RCC patients’ tissues and cells (HK-2, 786-O and A498). (d) Pearson’s correlation analysis analyzed the correlation between circ_0054537 and miR-640 levels in RCC tissues. (f) The predicted miR-640-binding sites in circ_0054537-WT were mutated. (g, h) Dual-luciferase reporter assay system compared luciferase activity of circ_0054537-WT/MUT vectors between miR-con mimic (miR-con) transfection and miR-640 mimic (miR-640) transfection in 786-O and A498 cells. (i, j) RIP assay detected RNA enrichment of circ_0054537 and miR-640 between anti-Ago2-mediated complex and anti-IgG-mediated complex. (k) RT-qPCR compared miR-640 level between si-con-transfected 786-O and A498 cells and si-circ_0054537 #1 -transfected above cells. *** P < 0.001

    Journal: Bioengineered

    Article Title: Silencing circular RNA circ_0054537 and upregulating microRNA-640 suppress malignant progression of renal cell carcinoma via regulating neuronal pentraxin-2 (NPTX2)

    doi: 10.1080/21655979.2021.1984002

    Figure Lengend Snippet: miR-640 was downregulated in RCC and correlated with circ_0054537 via targeting. (a, b) GSE61741 compared miRNAs levels including miR-640 in peripheral bloods between renal cancer patients and normal controls. (c, e) RT-qPCR detected miR-640 level in 39 RCC patients’ tissues and cells (HK-2, 786-O and A498). (d) Pearson’s correlation analysis analyzed the correlation between circ_0054537 and miR-640 levels in RCC tissues. (f) The predicted miR-640-binding sites in circ_0054537-WT were mutated. (g, h) Dual-luciferase reporter assay system compared luciferase activity of circ_0054537-WT/MUT vectors between miR-con mimic (miR-con) transfection and miR-640 mimic (miR-640) transfection in 786-O and A498 cells. (i, j) RIP assay detected RNA enrichment of circ_0054537 and miR-640 between anti-Ago2-mediated complex and anti-IgG-mediated complex. (k) RT-qPCR compared miR-640 level between si-con-transfected 786-O and A498 cells and si-circ_0054537 #1 -transfected above cells. *** P < 0.001

    Article Snippet: Protein G Sepharose 4 Fast Flow bead slurry (GE Healthcare, Beijing, China) was pre-coated with antibody against human Ago2 (anti-Ago2; ab32381, 1:50; Abcam) or the negative control anti-IgG (ab109489, 1:100; Abcam).

    Techniques: Quantitative RT-PCR, Binding Assay, Luciferase, Reporter Assay, Activity Assay, Transfection

    NPTX2 was upregulated in RCC and targeted by miR-640. (a) GEPIA showed NPTX2 expression on box plots in kidney renal clear cell carcinoma (KIRC, also named as ccRCC) tumor tissues and normal tissues. (b) RT-qPCR detected NPTX2 mRNA level in tissues from 39 RCC patients. (c) Pearson’s correlation analysis analyzed the correlation between miR-640 and NPTX2 mRNA levels in RCC tissues. (d, e) Western blotting detected PTX2 protein level in 39 RCC patients’ tissues and cells (HK-2, 786-O and A498). (f) The predicted miR-640-binding sites in NPTX2-3ʹUTR-WT were mutated. (g, h) Dual-luciferase reporter assay compared luciferase activity of NPTX2-3ʹUTR-WT/MUT vectors between miR-con transfection and miR-640 transfection in 786-O and A498 cells. (i, j) RIP assay detected RNA enrichment of miR-640 and NPTX2 mRNA between anti-Ago2-mediated complex and anti-IgG-mediated complex. (k) Western blotting compared NPTX2 protein level between in-miR-con transfection and in-miR-640 transfection in 786-O and A498 cells. ** P < 0.01 and *** P < 0.001

    Journal: Bioengineered

    Article Title: Silencing circular RNA circ_0054537 and upregulating microRNA-640 suppress malignant progression of renal cell carcinoma via regulating neuronal pentraxin-2 (NPTX2)

    doi: 10.1080/21655979.2021.1984002

    Figure Lengend Snippet: NPTX2 was upregulated in RCC and targeted by miR-640. (a) GEPIA showed NPTX2 expression on box plots in kidney renal clear cell carcinoma (KIRC, also named as ccRCC) tumor tissues and normal tissues. (b) RT-qPCR detected NPTX2 mRNA level in tissues from 39 RCC patients. (c) Pearson’s correlation analysis analyzed the correlation between miR-640 and NPTX2 mRNA levels in RCC tissues. (d, e) Western blotting detected PTX2 protein level in 39 RCC patients’ tissues and cells (HK-2, 786-O and A498). (f) The predicted miR-640-binding sites in NPTX2-3ʹUTR-WT were mutated. (g, h) Dual-luciferase reporter assay compared luciferase activity of NPTX2-3ʹUTR-WT/MUT vectors between miR-con transfection and miR-640 transfection in 786-O and A498 cells. (i, j) RIP assay detected RNA enrichment of miR-640 and NPTX2 mRNA between anti-Ago2-mediated complex and anti-IgG-mediated complex. (k) Western blotting compared NPTX2 protein level between in-miR-con transfection and in-miR-640 transfection in 786-O and A498 cells. ** P < 0.01 and *** P < 0.001

    Article Snippet: Protein G Sepharose 4 Fast Flow bead slurry (GE Healthcare, Beijing, China) was pre-coated with antibody against human Ago2 (anti-Ago2; ab32381, 1:50; Abcam) or the negative control anti-IgG (ab109489, 1:100; Abcam).

    Techniques: Expressing, Quantitative RT-PCR, Western Blot, Binding Assay, Luciferase, Reporter Assay, Activity Assay, Transfection